The In-Vitro Life-Cycle Of A Perkinsus Species (Apicomplexa, Perkinsidae) Isolated From Macoma-Balthica (Linneaus, 1758)

Using standard sterile techniques and a single medium previously described (Kleinschuster and Swink 1993), the in vitro culture of a Perkinsus species isolated from Macoma balthica was possible. Zoosporulation, the release of zoospores, and the reestablishment of secondary cultures from the zoospores completed an in vitro life cycle

Development of an in vitro culture method for cells and tissues from the zebra mussel (Dreissena polymorpha)

Despite the successful transfer of mammalian in vitro techniques for use with fish and other vertebrates, little progress has been made in the area of invertebrate tissue culture. This paper describes the development of an in vitro technique for the culture of both cells in suspension and tissue explants from the gill, digestive gland and mantle of the zebra mussel (Dreissena polymorpha) and their successful maintenance in culture for up to 14 days. Cell suspensions from the gills and digestive gland were the most successful technique developed with viability >80% maintained for up to 8 days in culture, suitable for use in short term toxicity tests. Tissue explants from the mantle were also maintained in culture for up to 14 days. This paper describes the challenges involved in the development of a novel in vitro culture technique for aquatic invertebrates